Particle in Cell-3D

Particle in Cell-3D is an ImageJ Macro designed to quantify the uptake of macro- and nanoparticles by cells.

Input

Routines 1, 2 and 3:

  • Confocal fluorescence image stack of a single cell with stained plasma membrane
  • Respective image stack of nanoparticles

Routines 4 and 5:

  • Confocal fluorescence image stack of nanoparticles

References

[1] Torrano A.A., Blechinger J., Osseforth C., Argyo C., Reller A., Bein T., Michaelis J., and Bräuchle C.
A fast analysis method to quantify nanoparticle uptake on a single cell level
Nanomedicine 8(11)(2013), 1815.[link]

[2] Blechinger J., Bauer A.T., Torrano A.A., Gorzelanny C., Bräuchle C., and Schneider S.W.
Uptake kinetics and nanotoxicity of silica nanoparticles are cell type dependent
Small 9(23)(2013), 3970.[link]

[3] Torrano A.A. & Bräuchle C.
Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy
Beilstein Journal of Nanotechnology 5 (2014), 1616.[link]

Contact Information

Adriano A. Torrano
Department of Chemistry and Center for NanoScience (CeNS)
University of Munich (LMU), Germany
Adriano.Torrano'at'gmail.com

Code

macro/particle_in_cell-3d.txt · Last modified: 2016/11/06 22:34 by aatorrano
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