ROI Color Coder

Colorizes selections listed in the ROI Manager by matching measurements to a color of a lookup table (LUT), generating particle-size heat maps. It complements the Analyze>Analyze Particles… command. ROI Color Coder is part of the BAR.

Keywords   Analyze particles, heat-map, lookup table, non-destructive image overlay, BAR


Output

blobs.gif in which the Area of segmented particles was matched to the fire LUT (filled ROIs) and Feret lengths to a custom heat-map LUT (contours)


Prompt

 Dialog prompt of ROI Color Coder


Requirements

ImageJ 1.46h or later. Use Help>Update ImageJ… to upgrade to a newer version.


Installation

  1. Subscribe to the BAR update site in Fiji. Alternatively, download the latest version of ROI_Color_Coder.ijm from GitHub (direct link).
  2. Place the file in the ImageJ/plugins/ or Fiji.app/plugins/ folder (or subfolder).
  3. The command ROI Color Coder will be listed in the Plugins> menu after running Help>Refresh Menus.

Tips

  • Rename your preferred LUTs in the ImageJ/luts/ directory with a numeric prefix (e.g. '00-Gold.lut') to have them listed first in the LUT dropdown menu
  • Check “Labels” in the ROI Manager to toggle ROI labeling. Adjust ROI labels using the ROI Manager More»Labels… command
  • Use the ROI Manager Flatten [F] command to obtain an RGB image with the overlay rendered as pixel data. The image overlay is also embedded when saving the image in PNG or JPEG format (File>Save As> submenu)
  • A zip folder containing several 'heat-map' LUTs can be downloaded from the ImageJ web site (direct link)
  • You can easily customize lookup tables using the Edit>Color>Edit LUT command.
  • The glasbey.lut (direct link) can be used to colorize selections randomly (although other strategies would probably perform better in this task, e.g., RoisToOverlay.js)
  • Once installed in the Plugins menu, ROI Color Coder can be called from other macros, e.g.:
    run("ROI Color Coder", "measurement=Area lut=Ice width=10 opacity=80");
  • See GitHub for more details



Change History

  • 2014.01.31 v.5.1
    • Added support for the Angstrom symbol
    • Moved file to GitHub
  • 2012.03.01 v.5
    • Min value is no longer restricted to zero
    • Legend is now fully customizable
  • 2010.12.06 v.4
    • Accepts all LUT files recognized by ImageJ (previous versions accepted only tab-delimited text LUT files)
    • Fixed an issue caused by files without extension saved in the luts directory
  • 2009.12.14 v.3
    • Added options for transparency, contours width, ROI filling
    • Results table is cleared and ROIs (re)measured if needed
  • 2009.12.01 v.2
    • Fixed labels misalignment in legend

Tiago Ferreira 2014/06/19 23:10

macro/roi_color_coder.txt · Last modified: 2014/06/19 23:11 by tferreira
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