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plugin:analysis:droplet_counter:start [2019/03/01 17:21]
ebmoll
plugin:analysis:droplet_counter:start [2019/03/01 17:29] (current)
ebmoll
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   y: 10um / 512 = 19.5nm   y: 10um / 512 = 19.5nm
   z: 2um / 30 = 66.7nm   z: 2um / 30 = 66.7nm
-This means the z-resolution is considerably worse than the x- and y-resolutions. (This is very common with CLSM images)+Normally, your imaging system gives you the width of a pixel (x and y values) and the stack step size (z value) directly. In our example the z-resolution is considerably worse than the x- and y-resolutions. (This is very common with CLSM images)
 **The z-ratio is then just z divided by x, i.e. 66.7nm/19.5nm = 3.4**. This is what should be put in the confusingly-named "Z/X aspect ratio" field. **The z-ratio is then just z divided by x, i.e. 66.7nm/19.5nm = 3.4**. This is what should be put in the confusingly-named "Z/X aspect ratio" field.
 That said, you can experiment a bit with this setting (set it to higher/lower values than the theoretically calculated ones) to get optimal object separation. That said, you can experiment a bit with this setting (set it to higher/lower values than the theoretically calculated ones) to get optimal object separation.
  
  
plugin/analysis/droplet_counter/start.txt · Last modified: 2019/03/01 17:29 by ebmoll
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