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plugin:analysis:figaro_psf_3d_optical_microscopy:start [2019/02/11 08:32]
lefortc [Protocol and Description]
plugin:analysis:figaro_psf_3d_optical_microscopy:start [2019/02/12 11:18] (current)
lefortc [Plugin FIGARO: 3D PSF estimation for optical microscopy]
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 ====== Plugin FIGARO: 3D PSF estimation for optical microscopy ====== ====== Plugin FIGARO: 3D PSF estimation for optical microscopy ======
  
-This plugin FIGARO helps tracking resolution of microscope systems by extracting dimensions and orientation of standardized microbeads images, acquired from test samples. In the development of optical microscopes for biomedical imaging, the evaluation of resolution is a fundamental parameter achieved by Point Spread Function (PSF) measurements. Sometimes, __**PSF measurement**__ procedure is not easy or impossible in case of **microspheres images presenting a high noise level**. The current method proposed into the plugin FIGARO is based on a variational approach for PSF modeling through multivariate Gaussian fitting, adapted to images highly damaged when aquisitions are realized in a high noise context. +This plugin FIGARO helps tracking resolution of microscope systems by extracting dimensions and orientation of standardized microbeads images, acquired from test samples. In the development of optical microscopes for biomedical imaging, the evaluation of resolution is a fundamental parameter achieved by Point Spread Function (PSF) measurements. Sometimes, __**PSF measurement**__ procedure is not easy or impossible in case of **microspheres images presenting a high noise level**. The current method proposed into the plugin FIGARO is based on a variational approach for PSF modeling through multivariate Gaussian fitting, adapted to images  acquired in a high noise context. 
 The plugin FIGARO is based on the __**analysis of a 3D image**__ of a single microbead and is **__adapted to any kind of optical microscopes__** generating 3D images of microbead.  The plugin FIGARO is based on the __**analysis of a 3D image**__ of a single microbead and is **__adapted to any kind of optical microscopes__** generating 3D images of microbead. 
-The plugin FIGARO have been validated for a __**PSF estimation of a multiphoton microscope**__.+The plugin FIGARO has been validated for a __**PSF estimation of a multiphoton microscope**__.
  
  
plugin/analysis/figaro_psf_3d_optical_microscopy/start.txt · Last modified: 2019/02/12 11:18 by lefortc
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