Use the commands in this submenu to analyze one-dimensional electrophoretic gels.
These commands are similar to the Gel Plotting Macros distributed with NIH Image.
Both use a simple graphical method that involves generating lane profile plots, drawing lines to enclose peaks of interest, and then measuring peak areas using the wand tool.
[insert image Gel]
Here is what you need to do to analyse a 1-D gel:
Added in v. 1.38n: Re-plot Lanes and Draw Lane Outlines commands and enabled “Invert Peaks” in the Gel Analyze Options dialog by default.
For practice, a sample gel is available in the File>Sample Images submenu. A tutorial is also available [insert link here] that shows how to use this procedure to analyze a dot blot.