This section documents the entries in the Graphical User Interface of ImageJ. The contents of these pages has been imported from the ImageJ website -written by Wayne Rasband- and updated where necessary. Last updated according to version 1.40b.
Creates a new image window or stack.
A dialog box allows you to specify the image title, type, dimensions and initial content.
Name is the title that will be used for the image Window. Type is the image type: 8-bit grayscale, 16-bit grayscale (unsigned), 32-bit (float) grayscale or RGB color. Fill With (White, Black, Ramp, or Clipboard Contents) specifies how the image is initialized. Width and Height specify the image dimensions in pixels. Width and Height are ignored if Fill With is set to Clipboard Contents. Set Slices to a value greater than one to create a stack.
Creates a new text window. The window is named Untitled.txt.
This command pastes the contents of the ImageJ clipboard as a new image.
This command pasted the contents of the operating system clipboard as a new image (requires Java 1.4 or later).
Reads an image and displays it in a separate window. Files must be in TIFF (uncompressed), PNG, GIF, JPEG, DICOM, BMP, PGM or FITS format. Also opens ImageJ and NIH Image lookup tables (with “.lut” extension). Additional file formats are supported via plugins installed in the Import submenu.
The Open Next command opens the next image in a sequence which is residing in the last open folder. The new image is not opened in a new window but rather replaces the current image. Press ALT to open the previous image.
This command allows to load sample images stored at the ImageJ site.
This submenu opens example images downloaded from the ImageJ Web site. The images can also be downloaded from http://rsb.info.nih.gov/ij/images/ and opened using File/Open.
This is useful to create sample macros (so they use the same image, regardless of where the macro is run).
For the DICOM (.dcm) images, press CTRL-i (Image/Show Info) to display the tags.
Opens a file kept in a history list of recently opened files.
The last 15 opened file names and paths are remembered for easy retrieval.
This submenu lists the installed image acquisition plugins.
Opens a sequence of images in a folder as a stack. The images must all be the same size and type. They can be in TIFF, JPEG, DICOM, BMP, GIF, FITS or PGM format, or in a format supported by the HandleExtraFileTypes plugin. There are two dialog boxes: one to select the folder and one to specify options. In the first dialog, select one of the images in the folder and click “Open”. Use the second (shown below) to specify which images to open, to reduce the image size, to convert to a different type, or to open the images as a “virtual” (disk-resident) stack.
Use the Number of Images field to specify how many images to open. Set Starting Image to n to start with the nth image in the folder. Set Increment to “2” to open every other image, to “3” to open every third image, etc. Enter a string into the File Name Contains field and ImageJ will only open files whose name contains that string.
Entering a value less than 100 in Scale Image reduces memory requirements. For example, entering 50 reduces the
amount of memory needed to open the stack by 75%.
Checking Convert to 8-bit Grayscale reduces memory requirements by 50% for 16-bit images and by 75% for 32-bit and RGB images.
Checking Convert to RGB allows a mixture of RGB and grayscale images to be opened.
Check Sort Names Numerically to open the stack in numeric file name order (e.g., “name1.tif”, “name2.tif”, “name10.tif”) instead of alphanumeric order (e.g., “name1.tif”, “name10.tif”, “name2.tif”). DICOM files in the same series (tag# 0020,0011) are always sorted by the image number tag (0020,0013). The “List Stack Tags” macro, part of the ListDicomTags macro set, lists the values of the image number and image series tags.
Check Use Virtual Stack to open the images as a read-only virtual (disk-resident) stack using a version of the Virtual Stack Opener plugin. This allows image sequences too big to fit in RAM to be opened, but access time is slower and changes
are lost when switching to a different image in the stack. Refer to the Process Virtual Stack macro for an example that shows how to process all the images in a virtual stack.
The size (width x height x depth) of the stack that will be created is displayed at the bottom on the dialog box.
Another way to open an image sequence is to use the OpenSeriesUsingFilter macro, which opens a series of images as a stack using a customizable file name filter.
Use this command to import images that are not in a file format directly supported by ImageJ. You will need to know certain information about the layout of the image file, including the size of the image, and the offset to the beginning of the image data.
Use the pop-up menu at the top of the dialog box to specify the image type. There are 13 choices:
Interleaved RGB images have pixels stored contiguously (rgbrgbrgb…) in a single image plane. Planar RGB images have the red, green and blue image data stored in separate 8-bit sample planes. ImageJ saves RGB images (both TIFF and raw) in interleaved format.
Image Width is the number of pixel in each row of image data and Image Height is the number of rows in the image. Offset to First Image is the number of bytes in the file before the first byte of image data.
Number of Images is the number of images stored in the file. If this value is greater than the actual number of images the resulting stack will get truncated to the actual size.
Gap Between Images is the number of bytes from the end of one image to the beginning of the next. Set this value to width x height x bytes-per-pixel x n to skip n images for each image read.
Check White is Zero if black pixels are represented using numbers that are less than the numbers used for white pixels. If your images look like photographic negatives, changing this field should fix the problem.
Little Endian Byte Order will probably need to be checked when importing 16-bit or 32-bit grayscale images from little-endian machines such as Intel based PCs.
Check Open All and ImageJ will import all the images in the folder as a stack. The images must all be the same size and type.
Opens an NIH Image lookup table, or a raw lookup table. The raw LUT file must be 768 bytes long and contain 256 reds, 256 blues and 256 greens. If no image is open, a 256×32 ramp image is created to display the LUT. Lookup tables with file names ending in “.lut” can also be opened using File/Open.
Opens a tab-delimited text file as a 32-bit real image. The image's width and height are determined by scanning the file and counting the number of words and lines. Use Image/Lookup Tables/Invert LUT to correctly display text files imported from NIH Image. For text files with integer values no larger than 255, use Image/Type/8-bits to convert to 8-bits. Before converting, disable “Scale When Converting” in Edit/Options/Miscellaneous to prevent the image from being scaled to 0-255.
Opens a text file. Right click (command-click on the Mac) in a text window to activate a pop-up menu with Save As, Cut, Copy, Paste and Select All commands. Most text files can also be opened using File/Open.
Opens an ImageJ Results table, or any tab or comma-delimited text file.
Downloads and displays a TIFF, ZIP compressed TIFF, GIF, JPEG or DICOM image specified by a URL. TIFF file names must end in “.tif”, ZIP file names must end in “.zip” and DICOM files names must end in “.dcm” (or “.zip” if compressed).
Here are some example URLs:
http://rsb.info.nih.gov/ij/images/clown.gif http://rsb.info.nih.gov/ij/images/ct.dcm file:///Macintosh HD/images/Nanoprobes.tif file:///D:\images\neuron.tif
Also, it opens a URL using the users default browser if the URL ends in “.html” or “/”.
run("URL...", "url=http://rsb.info.nih.gov/ij/"); run("URL...", "url=file:///Users/wayne/source/applet.html");
Imports a stack from a file containing a list of file names (from v. 1.39l).
Imports a Tiff virtual stack or HyperStack (from v. 1.39l).
Imports a EXIF data present in the headers of an open JPEG file.
Closes the currently active image window.
If the image has changed, you will be asked if you want to save the changes.
Saves the active image or stack in TIFF format.
To save only a selected area, create a rectangular selection and use the Image/Duplicate command.
Use this submenu to save the active image in TIFF, GIF, JPEG, “raw”, FITS, PGM or PNG format. Can also be used to save measurement results, lookup tables, selections, and selection XY coordinates.
Saves the active image or stack in TIFF format. TIFF is the only format (other than “raw”) that supports all ImageJ data types and the only format that saves spatial and density calibration.
Saves the active image in GIF format. RGB images must first be converted to 8-bit color using using the Image>Type>8-bit Color command. With v1.39 and later, stacks are saved as animated GIFs. Use Image>Stacks>Animation Options to set the frame rate.
Saves the active image in JPEG format. Use Edit/Options/JPEG Quality to specify the JPEG compression level (0-100). Lower values produce smaller files but poorer quality. Larger values produce larger files but better quality. Warning: the JPEG compressed format is lossy and results in artefacts. This format should not be used if you plan to make measurements on the image or do any further processing.
The Save as JPEG macro creates a command that allows the user to press “j' to set the compression level and save in JPEG format.
Saves the active image or stack as a TIFF file inside a compressed ZIP archive.
Saves the active image or stack as raw pixel data without a header.
* 8-bit images are saved as unsigned bytes,
* unsigned 16-bit images are saved as unsigned shorts
* signed 16-bit images (e.g., File>Open Samples>CT) are saved as signed shorts.
* 32-bit images are saved as floats and
* RGB images are saved in three bytes per pixel (24-bits interleaved) format.
16-bit and 32-bit (float) images are saved using big-endian byte order unless “Export Raw in Intel Byte Order” is checked in the Edit>Options>Input/Output dialog box.
Note that the option to save in Intel byte order requires ImageJ 1.37 or later.
Saves all the slices in the active stack as separate tiff, gif, jpeg or raw files.
Saves the active stack as an uncompressed AVI file, using the frame rate defined in the Image>Stacks>Animation Options dialog box.
Windows users can use VirtualDub to open uncompressed AVI files and resave as a compressed AVI. Macintosh users can use the QuickTime Movie Player program to open uncompressed AVI files and, with the Pro version, resave in compressed QuickTime format.
Saves the active image in BMP format.
Saves the active image in PNG format. This is a non-lossy compressed format.
Saves the active image in PGM format.
Saves the active image in FITS format. FITS stands for Flexible Image Transport System and is the most commonly used file format in astronomy. It saves 8-bit (by converting to 16-bit) and signed 16-bit (by converting to float) images.
Saves the active image's lookup table to a file. The 768 byte file consists of 256 red values, 256 green values and 256 blue values.
Saves the current area selection boundary to a file. Use File>Open to restore the selection.
Exports the XY coordinates of the line selection or area selection boundary as a two column, tab-delimited text file. Coordinates of rectangular, oval and straight line selections can not be exported. Use the Path Writer plugin plugin to save coordinates at evenly spaced one pixel intervals.
Exports the contents of the “Results” window as a tab-delimited text file.
Saves the active image as a spreadsheet compatible tab-delimited text file. For calibrated images and floating-point images, the “Decimal Places” field in Analyze>Set Measurements determines the number of digits to the right of the decimal point. For RGB images, each pixel is converted to grayscale using the formula:
gray = 0.299 * red + 0.587 * green + 0.114 * blue
or the formula:
gray = (red + green + blue) / 3
if “Unweighted RGB to Grayscale Conversion” is checked in Edit>Options>Conversions.
Replaces the active image with the last saved version.
This is a shortcut for closing the window without saving, and then reopening it. The command currently does not work with stacks.
The Page Setup dialog allows you to control the size of printed output, plus other printing options.
Scale values less than 100% reduce the size of printed images and values greater than 100% increase the size. 100% corresponds to 72 pixels per inch, about the unzoomed screen size of the image.
The size of the printed image is determined by the Scale value and the width and height of the image in pixels. Spatial calibration is ignored.
Check Draw Border to have ImageJ print a one pixel wide black border around the image.
Check Center on Page and the image will be printed in the center of the page instead of in the upper left corner.
Check Print Title to have the title of the image printed at the top of the page.
Check Selection Only to print the selection instead of the entire image.
Check Rotate 90° and the image will be rotated 90° to the left before being printed (ImageJ 1.33m or later).
Prints the active image.
The size of the printed image will normally be slightly less its size on the screen (unzoomed). Use the Page Setup dialog to increase of decrease the size of printed images. Images larger than the page are scaled to fit.
If you have not saved any images, you will be prompted to do so. You can also exit by clicking on the close button in the ImageJ window's title bar.