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macro:normalized_variance
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| + | ====== Normalized Variance ====== | ||
| + | **A macro to determine image focal quality image-wide (not ROI-wide).** | ||
| + | |||
| + | <code java> | ||
| + | |||
| + | // A macro to determine image focal quality image-wide (not ROI-wide) | ||
| + | // Based on algorithm F-11 "Normalized Variance" | ||
| + | // In: Sun et al., 2004. MICROSCOPY RESEARCH AND TECHNIQUE 65, 139–149. | ||
| + | // | ||
| + | // Version: 0.3 | ||
| + | // Date: 11/07/2006 | ||
| + | // Author: Andy Weller | ||
| + | |||
| + | macro "Normalized_Variance" { | ||
| + | run("Clear Results"); | ||
| + | run("Set Measurements...", " mean redirect=None decimal=5"); | ||
| + | run("Measure"); | ||
| + | mean = getResult("Mean"); | ||
| + | selectWindow("Results"); | ||
| + | run("Close"); | ||
| + | W = getWidth(); | ||
| + | H = getHeight(); | ||
| + | b = 0; | ||
| + | normVar = 0; // Set to 0 which is out of focus | ||
| + | for (j=0; j<H; j++) { | ||
| + | for (i=0; i<W; i++) { | ||
| + | p = getPixel(i,j); | ||
| + | t = (p-mean)*(p-mean); | ||
| + | b += t; | ||
| + | } | ||
| + | } | ||
| + | normVar = b/(H*W*mean); // Maximum value is best-focused, decreasing as defocus increases | ||
| + | print(normVar); // This can also (should) be changed to return(normVar) | ||
| + | } | ||
| + | |||
| + | </code> | ||
| + | |||
| + | --- //[[|Andy Weller]] 2008/10/24 11:45// | ||
macro/normalized_variance.txt · Last modified: 2019/04/12 11:13 (external edit)
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