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| + | ====== MetroloJ ====== | ||
| + | **This plugin helps keeping track of microscopes' performances by extracting 4 relevant estimators out of standardized images, acquired from test samples.** | ||
| + | ===== Author ===== | ||
| + | Fabrice P. Cordelières, Bordeaux Imaging Center (France). Fabrice.Cordelieres at gmail dot com | ||
| + | |||
| + | Cédric Matthews, CNRS - IBDML - UMR 6216,Marseille (France). cedric.matthews at ibdml.univmed.fr | ||
| + | |||
| + | This plugin has been created as part of the metrology workgroup work, within the framework of the [[http://rtmfm.cnrs.fr/|"French technological network for multidimensional fluorescence microscopies" (RT- MFM)]], supported by the [[http://www.mrct.cnrs.fr/|Mission ressources et compétences technologiques du CNRS" (MRCT)]]". | ||
| + | ===== Features ===== | ||
| + | This plugin allows measuring relevant parameters which helps testing, following and comparing microscopes performances. This is achieved by extracting four indicators out of standardized images, acquired from standardized samples: the estimation of the detector sensitivity , the evaluation of the field illumination homogeneity, the system resolution, and finally the characterization of its spectral registration. | ||
| + | |||
| + | ===== Description ===== | ||
| + | This plugin is composed of five parts: | ||
| + | * Generate CV report | ||
| + | * Generate field illumination report | ||
| + | * Generate PSF report | ||
| + | * Generate axial resolution report | ||
| + | * Generate co-alignement report | ||
| + | |||
| + | Please refer to the {{:plugin:analysis:metroloj:metroloj.pdf|plugin's manual}} for more details. | ||
| + | ===== References/Citation ===== | ||
| + | When using the “MetroloJ” plugin for publication, please refer to {{:plugin:analysis:metroloj:matthews_cordelieres_-_imagej_user_developer_conference_-_2010.pdf|Cédric Matthews and Fabrice P. Cordelieres, MetroloJ : an ImageJ plugin to help monitor microscopes' health, in ImageJ User & Developer Conference 2010 proceedings}}, to this webpage and of course to ImageJ, as explained in the [[http://rsbweb.nih.gov/ij/docs/faqs.html|FAQ section, on ImageJ’s website]]. A copy of your paper being sent to both our e-mail address would also be greatly appreciated ! | ||
| + | |||
| + | ===== Installation ===== | ||
| + | Simply download {{:plugin:analysis:metroloj:metroloj_.jar|}} to the Plugins folder of ImageJ, use the "Help/Update Menus" function to refresh the plugins' menu. | ||
| + | |||
| + | **Reports are generated as pdf files, using currently the {{:plugin:analysis:metroloj:itext-2.1.4.jar|iText library v2.1.4}} ([[http://itextpdf.com/download.php|Newer versions are available from iText website, but are not yet compatible with MetroloJ]]) which should be downloaded to the Plugins folder of ImageJ.** | ||
| + | |||
| + | <html><font color="red"><b>If you get an error message stating "java.lang.NoClassDefFoundError: com/lowagie/textDocumentationException...", it means you are missing the </b></font></html>{{:plugin:analysis:metroloj:itext-2.1.4.jar|iText library v2.1.4}}. <html><font color="red"><b>Download it and save it to your plugins' folder.</b></font></html> | ||
| + | |||
| + | ===== Downloads ===== | ||
| + | {{:plugin:analysis:metroloj:metroloj_.jar|The plugin is available from here}} | ||
| + | |||
| + | {{:plugin:analysis:metroloj:metroloj.pdf|The plugin's manual is available from here}} | ||
| + | |||
| + | ===== License ===== | ||
| + | This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 3 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program. If not, see [[http://www.gnu.org/licenses/|http://www.gnu.org/licenses/]]. | ||
| + | |||
| + | Copyright (C) 2011 Fabrice P. Cordelières/Cédric Matthews | ||
| + | |||
| + | ===== Changelog ===== | ||
| + | 22/04/11: First public release | ||
| + | |||
| + | 06/07/17: Correction of a bug in the field illumination report where wrong intensity was reported for the bottom-right corner | ||